Determination of the Contents of Evodiamine and Rutaecarpine in the Water Extract of Fructus Evodiae by HPLC

Objective To determine the contents of evodiamine and rutaecarpine in the water extract of Fructus Evodiae.MethodsThe samples were extracted with NH3-CHCl3 and the contents were determined by HPLC.The extrated fluid was performed on Agilent Zorbax Eclipse XDB-C18(4.6 mm×250 mm,5μm) column with the mobile phase of water-acetonitrile(49∶51) at a flow rate of 1.0 ml/min.The detection wavelength was set at 225 nm.The column temperature was 25℃ and the injection volumn was 20 μl.ResultsEvodiamine had a linear range in the contents of 0.2 ~4.0 μg/ml,R =0.999 7.The average recovery was 101.50% and RSD was 1.93%;Rutaecarpine had a linear range in the contents of 0.5~6.0 μg/ml,R=0.999 9.The average recovery was 100.80%,and RSD was 0.72%.ConclusionThe method is convenient,accurate and satisfying in separating evodiamine and rutaecarpine.It can be used in determination of evodiamine and rutaecarpine in the water extract of Fructus Evodiae.