Surveying the content of evodiamine,rutaecarpine and obaculactone in super instant drinking tablet of evodia rutaecarpine with HPLC

Objective To determine the content of evodiamine,rutaecarpine and Obaculactone with HPLC.Methods Hypersil ODS2 C 18 column(4.6 mm×250 mm,5μm);Shimadzu LC-20AD HPLC;mobile phase:acetonitrileWater-tetrahydrofuran-glacial acetic acid(34:64.7:1.1:0.2);flow rate:1.0 mL/min;detection wave-length:225 nm;column temperature:30℃.Results The amount of evodiamine,rutaecarpineand obaculactone was respectively at the ranges of 0.101 6～2.032μg(r 2 =0.999 9,n=6),0.097 4 ～ 1.948μg(r 2 =0.999 9,n=6),0.701 0～14.02μg(r 2 =0.999 9,n=6),which showed a good linear correlation between the ranges and the peak areas.The average recovery rate of evodiamine,rutaecarpine and obaculactone was 100.37%(RSD=1.29%,n=6),99.11%(RSD=0.83%,n=6) and 99.30%(RSD=0.52%,n=6).Conclusion The method is easy,fast,accurate and repeatable and should be used in the quality control of super instant drinking tablet of evodia rutaecarpine.