The effect of arsenic trioxide on cervical carcinoma and its relation with NDRG1

Objective To study the effect induced by arsenic trioxide(As2O3) on human cervical carcinoma HeLa cell lines.Methods HeLa cell lines were treated by As2O3.Cell proliferation was evaluated by MTT assay.Flow cytometry was used to examine apoptosis on cervical carcinoma HeLa cell lines.The expression of NDRG-1protein and mRNA were investigated by Western blot and RT-PCR techniques.Results MTT assay showed that As2O3 inhibited the growth of human cervical carcinoma HeLa cell lines in a dose-and time-dependent manner in vitro.Flow cytometry showed that As2O3 induced the apoptosis in a dose-and time-dependent manner(P0.05).Western-blot and RT-PCR techniques showed that As2O3 could up-regulate the expression of NDRG-1 protein and mRNA(P0.05).Conclusion As2O3 can significantly inhibit the growth and proliferation of HeLa cells and induce apoptosis of HeLa cells,which may be caused by up-regulating expression of NDRG-1 protein and mRNA.