The Effect Induced by Arsenic Trioxide on Human Cervical Carcinoma Hela Cell Lines

Objective To study the effect induced by 1.0,2.0,3.0 μmol/L arsenic trioxide(As2O3) on human cervical carcinoma Hela cell lines.Methods Hela cell lines were treated by 1.0,2.0,3.0 μmol/L As2O3.Cell proliferation was evaluated by MTT assay.Flow cytometry was used to examine apoptosis on cervical carcinoma Hela cell lines.The expression of Bcl-2 protein and Bax protein were investigated by immunohistochemical SABC techniques.Results MTT assay showed that As2O3 inhibited human cervical carcinoma Hela cell lines growth in vitro in a dose and time-dependent manner.Flow cytometry showed that As2O3 induced the apoptosis in a dose and time-dependent manner(P0.05).Immunohistochemical SABC techniques showed that As2O3 can downregulate the expression of Bcl-2 protein and upregulate the expression of Bax protein(P0.05).Conclusions As2O3 can significantly inhibit Hela cells growth and p roliferation and induce their apoptosis.The reason of these changes may be the downregulation expression of Bcl-2 protein and upregulation expression of Bax protein.