2008•Journal of Changshu Institute of TechnologyRequires access

Effect of Arsenic Trioxide on Human Cervical Squamous Carcinoma Cells in Vitro

Hui Chen

Abstract

By using light microscopy,fluorescence microscope,MTT reduction assay and flow cytometry,the apoptosis of HeLa cells treated with arsenict rioxide was detected.The cells treated with arsenict rioxide showed a typical apoptotic morphology.Arsenict rioxide could significantly inhibit the proliferation of HeLa cells in a dose-dependent manner.Data from flow cytometry showed that arsenict rioxide induced apoptosis of HeLa cells in a dose-dependent manner.Arsenict rioxide can induce HeLa cells apoptosis in a dose-dependent manner.

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Key concepts: HeLa, Flow cytometry, Apoptosis, Arsenic trioxide, MTT assay, In vitro, Fluorescence microscope, Chemistry