Study of Arsenic Trioxide on the Different Types of Cervical Cancer Cell Lines

Objective To observe the effects of As2O3 on the cervical cancer cell lines(Hela﹑SiHa).To study the mechanism of apoptosis and proliferation effect on cervical cancer cell lines by As2O3.Methods Cervical cancer cell lines of Hela and SiHa were cultured in vivo.ATP-bioluminescence Assay was given to analyze the cell proliferation effect.The cell morphologic features were investigated by HE(hematxylin and eosin) staining.The DNA fragmentation analysis was determined by the gel electrophoresis.The cell apoptosis ration was measured by flow cytometry(FCM).Cell immunohistochemical method was given to detect the expressions of apoptosis-related protein Fas and Fas ligand.Results ①The proliferation of cervical cell lines(SiHa、Hela) were inhibited effectively by different concentrations of As2O3(P0.05).②We can observe the typical apoptotic cells in the concentrations of 2.500 0 μmol/L and 5.000 0 μmol/L As2O3 after 48 h treatment.③As2O3(2.500 0 μmol/L,5.000 0 μmol/L) could significantly upregulate the expression of Fas on the two cell lines(P0.05),5.000 0 μmol/L As2O3 also up regulated the expression of FasL on Hela cell lines(P0.05).Conclusion As2O3 can inhibit the proliferation of the cervical cancer cell lines in vitro.Apoptosis does occur on cervical carcinoma cell lines induced by As2O3.The upregulation of Fas maybe one of the main mechanisms of apoptosis on cervical cancer cell lines by As2O3.