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2005•Zhongguo shiyan fangjixue zazhiRequires access

Determination of the Contents of Icariin in Gushen Pills with HPLC

Zhibin An

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Abstract

Objective:To establish the HPLC method for determination of icariin in Gushen pills.Method:HPLC was performed on a Diamonsil C_ 18 column (4.6×200mm,5μm) with acetonitrile-water(20∶80) as mobile phase.Detecting wavelength was 270nm and flow rate was 1.0mL/min.Result:A good linear relationship between peak area and concentration was noted at 0.15～2.10μg with a correlation coefficient of r=0.99998(n=6) for icariin.The recovery rate of icariin was 97.4%(RSD=2.17%).Conclusion:This method is accurate, repeatable and effective for quality control of Gushen pills.

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What this paper is about

Objective:To establish the HPLC method for determination of icariin in Gushen pills.Method:HPLC was performed on a Diamonsil C_ 18 column (4.6×200mm,5μm) with acetonitrile-water(20∶80) as mobile phase.Detecting wavelength was 270nm and flow rate was 1.0mL/min.Result:A good linear relationship between peak area and concentration was noted at 0.15～2.10μg with a correlation coefficient of r=0.99998(n=6) for icariin.The recovery rate of icariin was 97.4%(RSD=2.17%).Conclusion:This method is accurate, repeatable and effective for quality control of Gushen pills.

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Available abstract

Objective:To establish the HPLC method for determination of icariin in Gushen pills.Method:HPLC was performed on a Diamonsil C_ 18 column (4.6×200mm,5μm) with acetonitrile-water(20∶80) as mobile phase.Detecting wavelength was 270nm and flow rate was 1.0mL/min.Result:A good linear relationship between peak area and concentration was noted at 0.15～2.10μg with a correlation coefficient of r=0.99998(n=6) for icariin.The recovery rate of icariin was 97.4%(RSD=2.17%).Conclusion:This method is accurate, repeatable and effective for quality control of Gushen pills.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Chemistry, Correlation coefficient, Linear correlation, Linear relationship, Pill

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