Effect of Zhaqi Jiangtang Decoction on Expression of Renal NF-κB and Desmin in Diabetic Nephropathy Rats

Objective To investigate the effects of Zhaqi Jiangtang Decoction on the expression of renal NF-κB and Desmin in diabetic nephropathy(DN)rats.Methods The model rats of diabetic nephropathy were established by injecting streptozotocin(STZ)and then were randomly divided into different treatment groups by random number method.At the same time,normal control group was set up.After the establishment of the model,the relevant drugs were given intragastrically to different groups respectively.At the end of 10th week,the pathological changes of renal tissue were observed by HE staining under transmission electron microscope(TEM).The expression levels of renal nuclear factor kappa B(NF-κB) and Desmin were determined by Immunohistochemical method.Results There were significant pathological changes of diabetic nephropathy under the light microscope and TEM in the model group,and the expression levels of renal NF-κB and Desmin were significantly increased.The expression levels of renal NF-κB and Desmin were down-regulated in high-dose Zhaqi Jiangtang Decoction group and in glibenclamide group compared with the model group(P 0.05).Conclusion Zhaqi Jiangtang Decoction has protective effect on kidney damage in DN rats,and the mechanism may be achieved by reducing renal NF-κB and Desmin expression.