Effects of Zhaqi Jiangtang Decoction on the expression of TGF-β1,VEGF in kideny of diabetic nephropathy rats

AIM To investigate the effects of Zhaqi Jiangtang Decoction(Crataegi Fructus,Astragali Radix,Dioscoreae hypoglaucae Rhizoma,Maydis Stigma,Puerariae lobatae Radix,Salviae miltiorrhizae Radix et Rhizoma,Epimedii Folium,Scrophulariae Radix,etc.) on the expressions of transforming growth factor-β1(TGF-β1) and vascular endothelial growth factor(VEGF) in kidney of diabetic nephropathy rats.METHODS The model rats of diabetic nephropathy were established by injecting streptozotosin.After the model was formed,the relevant drugs were given intragastrically to different groups respectively.A group was medicated with glibenclamide as the control.After 10 weeks of treatment,the serum levels of blood urea nitrogen(BUN),creatinine(Scr) for treated diabetic nephropathy rats were detected.The pathological changes of renal tissue were observed by HE staining.The expressions of TGF-β1 and VEGF were determined by immunohistochemical.RESULTS Changes in biochemical indicators in the treatment groups were better than those in the model group.Compared with the model group,the downregulation of TGF-β1,VEGF was obvious in high-dose of Zhaqi Jiangtang Decoction group(P 0.05).CONCLUSION Zhaqi Jiangtang Decoction has protective effect to diabetic nephropathy rat with kidney damage,and the mechanism may be related to reduction in TGF-β1 and VEGF expressions.