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Determination of Ferulic Acid in Piantongning Tablet

Renqiang Ma

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Abstract

Objective To establish a method for the content determination of the Ferulic acid in the Piantongning Tablet. Methods HPLC was performed to determine the Ferulic acid on C18 column. The mobile phase was consisted of a mixture of CH3CN-0.085% phosphoric acid (20~80), the UV detection wavelength was 316 nm, and the temperature of column was 35~. Results The content of Ferulic acid showed a good linear relationship with peak area in the range of 2.8~42 μg/ml (r=0.9991), the average recovery was 97.6%, and RSD=1.1% (n=6). Conclusion This method is simple, sensitive, and accurate, and can be used for the quality control of the Piantongning tablet.

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What this paper is about

Objective To establish a method for the content determination of the Ferulic acid in the Piantongning Tablet. Methods HPLC was performed to determine the Ferulic acid on C18 column. The mobile phase was consisted of a mixture of CH3CN-0.085% phosphoric acid (20~80), the UV detection wavelength was 316 nm, and the temperature of column was 35~. Results The content of Ferulic acid showed a good linear relationship with peak area in the range of 2.8~42 μg/ml (r=0.9991), the average recovery was 97.6%, and RSD=1.1% (n=6). Conclusion This method is simple, sensitive, and accurate, and can be used for the quality control of the Piantongning tablet.

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Available abstract

Objective To establish a method for the content determination of the Ferulic acid in the Piantongning Tablet. Methods HPLC was performed to determine the Ferulic acid on C18 column. The mobile phase was consisted of a mixture of CH3CN-0.085% phosphoric acid (20~80), the UV detection wavelength was 316 nm, and the temperature of column was 35~. Results The content of Ferulic acid showed a good linear relationship with peak area in the range of 2.8~42 μg/ml (r=0.9991), the average recovery was 97.6%, and RSD=1.1% (n=6). Conclusion This method is simple, sensitive, and accurate, and can be used for the quality control of the Piantongning tablet.

Key concepts: Ferulic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Linear range, Linear relationship

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