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2010•Qilu Pharmaceutical AffairsRequires access

Determination of ferulic acid in Fufangyangjiao Tablets by HPLC

Qiang Li

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Abstract

OBJECTIVE To determine the ferulic acid in Fufangyangjiao Tablets.METHODS The HPLC analysis used Kromasil C18(4.6×250mm,5μm) as column,the mixture of methanol-0.1% acetic acid(30∶70) was used as mobile phase;The flow rate was 1.0ml·min-1,the detection wavelength was 321nm.RESULTS The linear range of ferulic acid was 0.08~0.28μg(r=0.9999),the average recovery was 100.3% with RSD 1.71%(n=6).CONCLUSION The method was simple and accurate,it can be used for the determination of ferulic acid in Fufangyangjiao Tablets.

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OBJECTIVE To determine the ferulic acid in Fufangyangjiao Tablets.METHODS The HPLC analysis used Kromasil C18(4.6×250mm,5μm) as column,the mixture of methanol-0.1% acetic acid(30∶70) was used as mobile phase;The flow rate was 1.0ml·min-1,the detection wavelength was 321nm.RESULTS The linear range of ferulic acid was 0.08~0.28μg(r=0.9999),the average recovery was 100.3% with RSD 1.71%(n=6).CONCLUSION The method was simple and accurate,it can be used for the determination of ferulic acid in Fufangyangjiao Tablets.

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Available abstract

OBJECTIVE To determine the ferulic acid in Fufangyangjiao Tablets.METHODS The HPLC analysis used Kromasil C18(4.6×250mm,5μm) as column,the mixture of methanol-0.1% acetic acid(30∶70) was used as mobile phase;The flow rate was 1.0ml·min-1,the detection wavelength was 321nm.RESULTS The linear range of ferulic acid was 0.08~0.28μg(r=0.9999),the average recovery was 100.3% with RSD 1.71%(n=6).CONCLUSION The method was simple and accurate,it can be used for the determination of ferulic acid in Fufangyangjiao Tablets.

Key concepts: Ferulic acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Linear range, Methanol, Linear relationship

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