2013•China Modern MedicineRequires access

The content determination of ferulic acid in Fukangning tablets by HPLC

Chen A-l

Abstract

Objective To establish a method for the content determination of ferulic acid in Fukangning tablets by HPLC.Methods SinoChrom ODS-BP C18 column(4.6 mm ×250 mm,5 μm) was used;the mobile phase consisted of acetonitrile-0.1% phosphate acid(22∶78) and the flow rate was 1.0 ml/min.The detection wavelength was 316 nm and the column temperature was at 25℃.Results Ferulic acid showed a good linear relationship(r=0.9998) in the range of 0.0014-0.0280 mg/ml with the addition recovery rate of 99.2% and RSD of 1.0%(n =6).Conclusion The method is simple and accurate with good repetition and high precision,which was suitable for the determination of ferulic acid in Fukangning tablets.

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Available abstract

Key concepts: Ferulic acid, Medicine, Chromatography, Content determination, High-performance liquid chromatography, Acetonitrile, Linear range, Linear relationship