Callus Induction and Differentiation of Mature Embryos Derived from Different Wheat Varieties

Mature embryos from four wheat varieties were cultured in different media to study the induction of the callus and plants regeneration. The results indicated that different varieties showed significantly different responses under various culture conditions. The most suitable concentration of 2,4-D for callus induction of mature embryo from four wheat varieties was different. The optimal 2,4-D concentration was 2.0 mg / L for Shi4185 and Kemai NO.1,and 4.0 mg / L for Yangmai 12 and Bobwhite,separately. The best condition for callus redifferentiation also varied. MS medium supplemented with 2.0 mg / L KT and0.1 mg / L NAA for Shi4185 and Bobwhite,1.0 mg / L KT and 0.5 mg / L NAA for Kemai NO.1,and 1.0 mg / L KT and 0.1 mg / L NAA for Yangmai 12 was conducive to improving differentiation and regeneration, respectively. In addition, with the extension of culture time,the differentiation rate of callus of wheat mature embryos would decline to different degree.