Induction and Differentiation of Calli from Mature Wheat Embryos

[Objective] The study aimed to screen the wheat variety with strong regeneration ability and establish the high effective and high frequency plant regeneration system for wheat mature embryos.[Method] With the mature embryos of wheat varieties Kangdongzao-30,Baimai 4411 and 06-4046 as the explants,MS was as the basic medium and was added by the different hormones to match the different media for induction,subculture,differentiation and rooting and some factors effecting the induction and differentiation of wheat callus were discussed.[Result] When 2,4-D was in 1-5 mg/L,its induction had little difference on the callus induction for 3 wheat varieties,with all the induction rate of above 80%.2,4-D at 2 mg/L was more advantageous to induce the embryogenic callus,with the induction rate of above 90%.Adding 2 mg/L KT in differentiation medium was useful to improve differentiation and regeneration of Kongdongzao-30 and Baimai 4411 and adding 0.5 mg/L KT in differentiation medium was more advantageous to the differentiation of mature embryo and the regeneration seedlings of 06-4046.[Conclusion] Adding 2 mg/L 2,4-D and some KT in MS medium could promote the callus induction of wheat mature embryos and the differentiation of regeneration seedlings.