2006•Food ScienceRequires access

Detection of Hydroxy Safflor Yellow A in Health Foods by RP-HPLC

Wang He-bin

Abstract

To determine the content of hydroxysafflor yellow A in the health foods by RP-HPLC, the hydroxysafflor yellow A could be separated on Kromasil-C 18 with nonlinearity gradient elution of CH 3OH-HAc(22:78) at a flow -rate of 1.0ml/min and also assayed at UV403nm, with the column temperature 25 ℃. The average recovery of sample was 98.5% 。This method was simple, rapid, accurate and reiable. It is suitable for the determination of HSYA concentration in health foods.

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Key concepts: Chromatography, Gradient elution, Chemistry, High-performance liquid chromatography, Health food, Elution, Food science