Expressions of human papilloma virus 16/18,Bcl-2 and Survivin in cervical cancer tissue

Objective To study the expressions and significance of human papilloma virus( HPV) 16 /18,Bcl-2 and Survivin in cervical cancer tissue. Methods Eighty cervical cancer tissue specimens,seventy cervical intraepithelial neoplasia(CIN) tissue specimens and thirty-two normal cervical tissue specimens in Hubei Cancer Hospital were collected from April2010 to July 2012. The expressions of HPV16 /18 and Survivin in the three tissues were detected by hybridization in situ. The expression of Bcl-2 was detected by immunohistochemistry. The relationship between the expressions of HPV16 /18,Bcl-2,Survivin and the clinical pathological characteristics of cervical cancer was analyzed. Results The positive rate of Survivin mRNA in normal cervical tissue,CIN and cervical cancer tissue was 6. 25%,45. 71% and 77. 50%,respectively. The positive rate of Bcl-2 protein in normal cervical tissue,CIN and cervical cancer tissue was 3. 13%,40. 00% and 71. 25%,respectively. The positive rate of HPV16 /18 in normal cervical tissue,CIN and cervical cancer tissue was 3. 13%,51. 43% and 83. 75%,respectively. There was significant difference in the positive rate of HPV16 /18 DNA,Bcl-2 and Survivin mRNA in the three kinds of tissues(P 0. 01). The expressions of Survivin mRNA,Bcl-2 and HPV16 /18 DNA in CIN tissues were correlated to CIN stages. The positive expressions of Survivin mRNA,Bcl-2 and HPV16 /18 DNA in CIN Ⅲ were obviously higher than those in CINⅠ and Ⅱ(P 0. 01). The expression of Bcl-2 protein was correlated to differentiation degree of cervical cancer and the lymph node metastasis(P 0. 01). But there was no significant correlation between the expression of Bcl-2 protein and tissue type,clinical stage,tumor growth type of cervical cancer(P 0. 05). The expressions of Survivin mRNA and HPV16 /18 DNA were correlated to differentiation degree,clinical stage and lymph node metastasis of cervical cancer(P 0. 01,P 0. 05). But the expressions of Survivin mRNA and HPV16 /18 DNA were uncorrelated to tissue type,tumor growth type of cervical cancer(P 0.05). Conclusions The high expressions of HPV16/18,Bcl-2 and Survivin were probably correlated to the occurrence and development of cervical cancer. The detections of HPV16 /18,Bcl-2 and Survivin were helpful for the early diagnosis,curative effect evaluation of operation and prognosis judgement of cervical cancer.