2005•Jilin Agricultural SciencesRequires access

Transformation of Herbicide Resistant Gene to Rice via Biolistic Method

Jingyu Wang

Abstract

A herbicide gene(bar) was delivered into a commercial rice variety Changbai 8 cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun(PDS-1000/He) transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants (D0-1, D0-2 and D0-3) showed herbicide resistance. Gene silence was observed in one transgenic rice plant (D0-5).

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Key concepts: Transformation (genetics), Genetically modified rice, Callus, Genetically modified crops, Biology, Gene gun, Japonica rice, Gene