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Transgenic Rice Plant Obtained from Introduction of Herbicide Resistant Gene bar into Rice by Pollen-tube Pathway Method

Wang Cai-lin, Zhao Ling, Zong Shouyu, LÜ Chuan-gen, Zou Jiang-shi, Weimin Zhu, Xiaolan He, Junqi Huang, Zhen-zhen Gong

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Abstract

Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line E32 by pollen-tube pathway method. The transgenic rice plant was proved to have expressed good resistance to herbicide Basta. Analysis of PCR proved the integration of bar gene into the genome of the transgenic plant. Genetic analysis indicated that the bar gene was genetically delivered to the progeny. Breeding lines with consistent resistance were segregated in T 3 generation.

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What this paper is about

Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line E32 by pollen-tube pathway method. The transgenic rice plant was proved to have expressed good resistance to herbicide Basta. Analysis of PCR proved the integration of bar gene into the genome of the transgenic plant. Genetic analysis indicated that the bar gene was genetically delivered to the progeny. Breeding lines with consistent resistance were segregated in T 3 generation.

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Available abstract

Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line E32 by pollen-tube pathway method. The transgenic rice plant was proved to have expressed good resistance to herbicide Basta. Analysis of PCR proved the integration of bar gene into the genome of the transgenic plant. Genetic analysis indicated that the bar gene was genetically delivered to the progeny. Breeding lines with consistent resistance were segregated in T 3 generation.

Key concepts: Genetically modified rice, Transgene, Biology, Genetically modified crops, Pollen, Pollen tube, Gene, Herbicide resistance

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