Transgenic Rice Plants Conferred Herbicide ResistanceObtained by Particle Bombardment

Herbicide resistant gene was delivered into rice (japonica) embryogesis callus induced from mature rice embryo via particle bombardment method. Six transgenic plants were obtained. Parameters affecting transformation efficiency were studied by gus transient expression. When the distance between DNAgold pellet and the target cell is 9 cm, bombarded for 2 times per dish, the transformation efficiency can be as high as 90%, and 100～200 blue spots can be detected on the callus. It was demonstrated that the foreign gene had been inserted into rice genome and expressed in transgenic rice plant by gus biochemical analysis, spraying herbicide on the leaf and PCR analysis.