2004•Shenyang Nongye Daxue xuebaoRequires access
Trcensformation of herbicide-resistant gene bar into Japonica rice with restoring gene mediated by A. Tumefaciens
Lili Shi, Sun Zongxiu, Songwen Wang, Cai BaoLi, Xin Zhang, Xia Liu, Guo Yu-hua
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Abstract
Two japonica rice(Oryza sativa L.)varieties Jindao107 and Jindao18 with restoring gene were used for the genetic transformation by using Agrobacterium tumefaciences EHA105 harboring binary vector containing Bar gene and selectable marker gene of HPT. 77 and 55 transgenic rice lines were obtained respectively. Spraying of 0.25% Basta to T0 transgenic plants, 92% and 78% of them have behaved resistance to herbicide Basta. PCR analysis showed that 400bp distinctice band was multiplied, indicating that the Bar gene had been integrated into the rice genome. The segregation of HPT gene in most of T1 progeny corresponded to the Mendelian ratio of 3∶1.