Study on Simultaneous Determination of 13 Sulfonamides Residues in Aquatic Products by HPLC

A high performance liquid chromatographic method has been developed for the simultaneous determination of thirteen sulfonamides residues in aquatic products(eels, longsnout catfish and langouste). The samples were extracted with acetonitrile, then cleaned up by n-hexane liquid-liquid extraction and solid phase extraction. The residues were determined by high perfor- mance liquid chromatography with ultraviolet detection and quantified by external standard method. The detection limits were 0.02mg/kg for sulfamethazine (SM2), sulfameter (SMT), sulfamonomethoxine(SMM), sulfachloropyridazine(SCP), sulfamethoxazole(SM-Z), sulfisoxazole(SIX), sulfadimethoxine(SDM) and sulfaquinoxaline(SQ), and 0.05 mg/kg for sulfadiazine(SD), sulfapyridine(SPD), sulfathiazole(ST), sulfamerazine(SM1) and sulfamethizole(STZ). The linear ranges were 0.1～5.0μg/ml. The average recoveries in spiked sample were 62%～97%.