Optimization of testing conditions for determination of sulfonamides residues in aquatic products by post-column derivation high performance liquid chromatography

The testing conditions were optimized base on the standard of determinating eight sulfonamides in aquatic products by post-column derivation high performance liquid chromatography(HPLC) method.In the optimized conditions,eight sulfonamides(sulfadiazine,sulfathiazole,sulfamerazine,sulfamethazine,sulfamethoxazole,sulfamethoxypyridazine,sulfisoxazole,sulfaquinoxaline) residues in aquatic products were separated and detected.The calibration curves were linear in a concentrations range of 0.025~0.5 μg/mL for sulfadiazine,sulfathiazole,sulfamerazine,sulfamethazine;0.05~1.0 μg/mL for sulfamethoxazole,sulfamethoxypyridazine,sulfisoxazole and 0.1~2.0 μg/mL for sulfaquinoxaline,with r 0.9990.The average recovery was 84.2%~109% and the relative standard deviation was in the range of 1.64%~10.6% for spiked weever sample with 5 μg/kg~100 μg/kg of sulfonamides.