ScholarLens Back to search
2008•Jiangsu nongye xuebaoRequires access

RAPD Analyses of Mitochondrial DNAs of CMS Pepper Line and Its Maintainer Line

Shubin Wang, Luo XiangDong, Liangfang Dai, Jinfeng Chen, Jinbing Liu, Pan BaoGui

Open publisher page 0 citations

Abstract

The mitochondria and mtDNAs were extracted from chloresis pepper seedlings at 10-15 d of CMS pepper 21A and its maintainer 21B.The mitochondria of pepper stained by PTA under the transmission electron microscrope showed intact shape,ellipsoid and less impurity.Electrophoresis detected quite pure mitochondrial DNA with no degeneration and concentration of 20-50 ng/μl which could be used for RAPD analysis.100 random primers were used for analyzing the difference of mtDNA between CMS 21A and its maintainer 21B by means of RAPD technique.Among them 8 primers presented polymorphism.Furthermore,the specific RAPD marker CMSAG3430 related to CMS gene was found.

About this research paper

What this paper is about

The mitochondria and mtDNAs were extracted from chloresis pepper seedlings at 10-15 d of CMS pepper 21A and its maintainer 21B.The mitochondria of pepper stained by PTA under the transmission electron microscrope showed intact shape,ellipsoid and less impurity.Electrophoresis detected quite pure mitochondrial DNA with no degeneration and concentration of 20-50 ng/μl which could be used for RAPD analysis.100 random primers were used for analyzing the difference of mtDNA between CMS 21A and its maintainer 21B by means of RAPD technique.Among them 8 primers presented polymorphism.Furthermore,the specific RAPD marker CMSAG3430 related to CMS gene was found.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The mitochondria and mtDNAs were extracted from chloresis pepper seedlings at 10-15 d of CMS pepper 21A and its maintainer 21B.The mitochondria of pepper stained by PTA under the transmission electron microscrope showed intact shape,ellipsoid and less impurity.Electrophoresis detected quite pure mitochondrial DNA with no degeneration and concentration of 20-50 ng/μl which could be used for RAPD analysis.100 random primers were used for analyzing the difference of mtDNA between CMS 21A and its maintainer 21B by means of RAPD technique.Among them 8 primers presented polymorphism.Furthermore,the specific RAPD marker CMSAG3430 related to CMS gene was found.

Key concepts: RAPD, Software maintainer, Pepper, Mitochondrial DNA, Cytoplasmic male sterility, Biology, Gene, Mitochondrion

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RAPD Analyses of Mitochondrial DNAs of CMS Pepper Line and Its Maintainer Line — Research Paper | ScholarLens