Prevention and cure effect of Danhuangfang on acute hepatic failure rats thioacetamide-induced

Objective:To explore the effects and probable mechanism of Danhuangfang(DHF) on rats with laboratory acute hepatic failure.Methods: The rats were injected with thioacetamide(TAA) 600mg/kg twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the normal saline hypodermically.The rats were treated with the normal saline,DHF low,middle and high dosages,PHGF respectively every day for 3 days before TAA were injected.To observe the mortality of the rats in 48 hours,and 48 hours later,the blood were collected to measure the indexes of ALT、AST、TBil、tumor necrosis factor(TNF-α)、interleukin-6(IL-6),and at the same time,pathological morphologies of hepatic tissues were observed respectively.Results: The DHF treatment group was super to controlled group in the mortality and the indexes reducing ALT、AST、TBil、TNF-α、IL-6,improving liver tissue pathological changes of rats with acute hepatic failure.Comparing with model group and control group,there are significant difference(P0.05 or P0.01).Conclusion: The DHF have resistance against the rat with acute hepatic failure by TAA,it related to decrease TNF-α the important medium of acute hepatic failure and IL-6.