Effects of Kangganshuai Fufang on the expression of HMGB1 protein and PCNA protein in rats with acute hepatic failure

Objective:To observe the effects of Kangganshuai Fufang(KGSFF) on the expression of HMGB1 and PCNA and to explore its mechanism related to relieve the damage and promoting regeneration in rats with laboratory acute hepatic failure.Methods:One hundred and ten Wistar male rats were randomly divided into six groups:normal control group(n=10),model control group(n=20),PHGB control group(n=20),YCHT group(n=20),KGSFF group of small dose(n=20),KGSFF group of large dose(n=20).All rats were injected with D-GalN 800mg/kg and LPS 0.1mg/kg except the normal group injected with the salt solution intraperitoneally.In 2 hours after model being made,the rats of nomal control group and model control group were treated with salt solution,KGSFF low and high dosages,YCHT,PHGF were treated respectively at a dosage of 1.5ml/100g twice every day for 3 days.And 72 hours later,the blood and hepatic tissues were collected.The mortality of the rats were observed in 3 days.The liver function including serum level of ALT,AST and TBil were measured by biochemical methods.The pathological morphologies of hepatic tissues were examined with HE staining.The expression of HMGB1 protein in hepatic tissues were analyzed by Western blotting.The expression of PCNA protein in hepatic tissues were showed with immunohistochemical staining.Results:Compared with those in the normal rats,the mortality of the rats and serum ALT,AST,TBil level remarkablely inereased,the expression of PCNA and HMGB1 in hepatic tissue significantly deceased,and cell necrosis and inflammatory infiltration were more obvious in the model rats(all P0.01).While in the KGSFF group of large dose,the cell necrosis and inflammatory infiltration were significantly improved,the expression of PCNA in hepatic tissue significantly deceased,and changes of the other above-mentioned inexes were significantly reversed(P0.01 or P0.05).Conclusion:Immunoreaction damage induced by HMGB1 plays a role in AHF.The KGSFF has resistance against the rats with acute hepatic failure,and its mechanism may be related to decreasing of HMGB1 which is the important medium of relieving the intestinal endotoxemia.The KGSFF can develop the regeneration of hepatic cells.