Effects of Kangganshuai Fufang on protective function and expression of TNF-alpha in rats with acute hepatic failure

Objective:To observe the effects of Kangganshuai Fufang(KGSFF)on the protective function and the expression of TNF-α and to explore its mechanism related to relieve the damage and promoting regeneration in rats with laboratory acute hepatic failure.Methods:One hundred and ten Wistar male rats were randomly divided into six groups: normal control group(n=10),model control group(n=20),PHGF control group(n=20),YCHT group(n=20),KGSFF group of small dose(n=20),KGSFF group of large dose(n=20).All rats were injected with D-GalN 800mg/kg and LPS 0.1mg/kg except the normal group injected with the salt solution intraperitoneally.In 2 hours after model being made,the rats of nomal control group and model control group were treated with salt solution,KGSFF low and high dosages,YCHT,PHGF were treated respectively at a dosage of 1.5ml/100g twice every day for 3 days.And 72 hours later,the blood and hepatic tissues were collected.The mortality of the rats were observed in 3 days.The liver function including serum level of ALT,AST,TBil and TNF-α were measured by biochemical methods.The pathological morphologies of hepatic tissues were examined with HE staining.The level of TNF-α in hepatic tissues were measured by immunohistochemical staining.Results:Compared with those in the model rats,the mortality of the rats and serum ALT,AST,TBil and TNF-α level remarkablely decreased,the cell necrosis infiltration were significantly improved,and the level of TNF-α in hepatic tissues also droped in the KGSFF group of large dose(P0.01 or P0.05).Conclusion:The KGSFF has an outstanding protective function on the rats with acute hepatic failure,and its mechanism may be related to decreasing TNF-α,relieving the intestinal endotoxemia and improving the liver function.