Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β_7 gene

Objective:To clone and analyze a full-length cDNA encoding mouse integrin β7,and repair the mutation of β7 cDNA that caused the change of amino acids. Methods:The cDNA of β7 gene was amplified by RT-PCR using the total RNA as extracted from mouse small intestine Peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed E.coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of β7 cDNA that caused the change of amino acids was repaired. Results:The cDNA of mouse β7 has an complete open reading frame with a length of 2418 bp,which encodes a product of 806 amino acid,and has 10 base pairs mutation of β7 gene and 5 base pairs mutation that caused the change of amino acids was repaired. Conclusion:The cDNA of mouse β7 was cloned successfully,which posed a basis for further researching on its biological function.