The effects of Alarelin combined with DDP on apoptosis in the drug resistant human ovarian cancer cell Line CoC1/cDDP

Objective To investigate the effect and the mechanisms of Alarelin combined with cisplatin(DDP)on the apoptosis in drug-resistant human ovarian cancer cell line CoC1/cDDP.Methods CoC1/cDDP cell were treated with different concentrations of alarelin,DDP and alarelin+DDP.The survivin-Ex3mRNA,caspase-3 mRNA were measured by RT-PCR.The G1cells and apoptosis cells and the cell mitochondrial membrane potential(ΔΨm)were measured by FCM.The growing inhibiting rates were examined by MTT.Results ① The apoptosis cells and the growing inhibiting rates increased with the concentration of DDP increased(P0.05).Alarelin increased the percentages of sub-G1and AnnexinV+/PI-cells in a dose-dependent manner(P0.01).Compared with separate use alarelin or DDP,after treating with alarelin+10μg/ml DDP,the apoptosis cells increased more obviously.The percentages of sub-G1,AnnexinV+/PI-cells and the percentages of Rh123cells were increased(P 0.01).②Alarelin significantly increased the expression of caspase-3mRNA in a dose-dependent manner(P 0.01),while treating with alarelin or alarelin combined with DDP,the expression of survivin-Ex3mRNA had no significant increased(P0.05).Conclusions Alarelin has strong reversal effects on DDP resistance in CoC1/cDDP,the effects may be related to decreasing theΔΨm and increasing expression of caspase-3mRNA.