2005•Research and Practice on Chinese MedicinesRequires access

Determination of Puerarin in Xintongping Granules by RP-HPLC

Huang Gui-ping

Abstract

Objective To establish a method for the determination of puerarin in Xintongping Granules.Methods HPLC method was developed for the determination of puerarin in Xintongping Granules on Alltima C_(18)column(5 μm,4.6 mm×250 mm),with methanol-0.3% phosphoric acid(26∶74/v∶v) as a mobile phase(1.0 mL·min~(-1)) and detecting wavelength at 250 nm.Results The linear range of puerarin was from 0.041 8 μg～1.003 2 μg and the correlation coefficient was 0.999 9.The average recovery rate was 99.6%,RSD was 1.8%(n=6).Conclusion The method is convenient and accurate,and can be used control the granule's quality.

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Key concepts: Puerarin, Chromatography, Chemistry, Phosphoric acid, Granule (geology), High-performance liquid chromatography, Correlation coefficient, Linear correlation