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2003•Unpublished venueRequires access

Cloning and Sequencing of Mouse IL-18 and Constructionof Recombinant Plasmid pIRES-IL18-B7.1

Jin Guang, MH Radiobiology

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Abstract

Objective: To clone the sequence of the cDNA of mouse IL 18 coding area and construct its expression vector. Methods: Using mouse splenocyte mRNA as template to obtain full length mIL 18 with the technique of RT PCR followed by automatic sequencing of pMD18T mIL 18 and to construct a recombinant plasmid containing mIL 18 and B7.1 genes with recombinant DNA technique. Results: Sequencing proved the cloned mIL 18 cDNA to be completely identical with that reported in the literature and the recombinant plasmid containing mIL 18 and B7.1 was constructed successfully. Conclusion: mIL 18 cDNA was successfully cloned and an expression vector pIRES IL18 B7.1 was constructed.

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What this paper is about

Objective: To clone the sequence of the cDNA of mouse IL 18 coding area and construct its expression vector. Methods: Using mouse splenocyte mRNA as template to obtain full length mIL 18 with the technique of RT PCR followed by automatic sequencing of pMD18T mIL 18 and to construct a recombinant plasmid containing mIL 18 and B7.1 genes with recombinant DNA technique. Results: Sequencing proved the cloned mIL 18 cDNA to be completely identical with that reported in the literature and the recombinant plasmid containing mIL 18 and B7.1 was constructed successfully. Conclusion: mIL 18 cDNA was successfully cloned and an expression vector pIRES IL18 B7.1 was constructed.

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Available abstract

Objective: To clone the sequence of the cDNA of mouse IL 18 coding area and construct its expression vector. Methods: Using mouse splenocyte mRNA as template to obtain full length mIL 18 with the technique of RT PCR followed by automatic sequencing of pMD18T mIL 18 and to construct a recombinant plasmid containing mIL 18 and B7.1 genes with recombinant DNA technique. Results: Sequencing proved the cloned mIL 18 cDNA to be completely identical with that reported in the literature and the recombinant plasmid containing mIL 18 and B7.1 was constructed successfully. Conclusion: mIL 18 cDNA was successfully cloned and an expression vector pIRES IL18 B7.1 was constructed.

Key concepts: Complementary DNA, Recombinant DNA, Plasmid, Molecular biology, Cloning (programming), Biology, clone (Java method), Coding region

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