Cloning and sequencing of mouse B7-2 gene and construction of its recombinant plasmids

Objective To clone the sequence of the cDNA of mouse B7 2 gene and construct its expression vectors. Methods Using mouse splenocyte mRNA as template to obtain full length B7 2 with the technique of RT PCR followed by automatic sequencing of pMD18T B7 2 and to construct recombinant plasmids containing CMV, Egr 1 and B7 2 gene with recombinant DNA technique. Results Sequencing proved the cloned B7 2 cDNA to be essentially identical with that reported in the literature and the recombinant plasmids containing CMV, Egr 1 and B7 2 gene were constructed successfully. Conclusion B7 2 cDNA was successfully cloned and two expression vectors pcDNA3 1 CMV B7 2 and pcDNA3 1 Egr B7 2 were constructed.