Molecular cloning of mouse IL-17 gene and construction of its eukaryotic expression vector.

Objective To clone the mouse IL-17 gene and construct its eukaryotic expression vector.Methods The cDNA encoding mIL-17 was amplified by RT-PCR using the total RNA extracted from spleen cells Of C57BL/6 mice immunized with LPS in CFA.The PCR product was cloned into pcDNA3.1 vector and then transformed into E.coli DH5α.The positive recombinant clone was analyzed by PCR,digestion of restriction endonuclease and DNA sequencing.Results The recombinant pcDNA3.1-mIL-17 had a complete open reading frame of mIL-17 and shared 100% homology with the sequence of mRNA for mIL-17 in gene bank.Conclusion The cDNA of mIL-17 is cloned successfully,which will be helpful for the further research on its biological function in autoimmune.