Clone and identification of human B7-1 cDNA

Objective: To construct recombinant B71 retrovirus expressing vector by clone of B7-1 gene from human blood and construct the recombinant plasmid of B7-1.Method: Primers for B7-1 were designed and synthesized according to the sequence of human B7-1 gene derived from GenBank.The full length cDNA of B71 was cloned by RT-PCR techniques.The recombinant plasmid pGEM-T-B7 were constructed by DNA recombinant techniques.Results: The length of RT-PCR product coincided with that of authors′ anticipation(889 bp),and the recombinant plasmid was confirmed by restriction enzyme digesting with EcoRI and Hind III.The sequencing result of the cDNA was identical to the sequence of B7-1 cDNA in GenBank,and the full length cDNA of human B7-1 was successfully inserted into the pGEM-T vector.Conclusion: The successful cloning of human B7-1 cDNA,as well as the construction of its retrovirus expressing vector enables us to further investigate the role of B7-1 in tumor immunogene therapy.