2010Journal of Zhengzhou University of Light IndustryRequires access

Research on enzymatic extraction processing of total flavone form Morifolium Ramat Chrysanthemum

Chang Fa-ling

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Abstract

The optimum conditions of enzymatic extraction total flavone from Morifolium Ramat Chrysanthemum were determined by orthogonal test.The results showed that the ratio of material and solvent is 1∶10,enzyme treatment temperature was 45 ℃,pH was 6.2,cellulase dosage was 0.25 g/L,pectase dosage was 0.3 g/L,hydrolysis time was 150 min,extraction time was 60 min,volume of extracting solution was 40 mL.Under the conditions,the extraction rate of total flavone is up to 6.83%.

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What this paper is about

The optimum conditions of enzymatic extraction total flavone from Morifolium Ramat Chrysanthemum were determined by orthogonal test.The results showed that the ratio of material and solvent is 1∶10,enzyme treatment temperature was 45 ℃,pH was 6.2,cellulase dosage was 0.25 g/L,pectase dosage was 0.3 g/L,hydrolysis time was 150 min,extraction time was 60 min,volume of extracting solution was 40 mL.Under the conditions,the extraction rate of total flavone is up to 6.83%.

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Available abstract

The optimum conditions of enzymatic extraction total flavone from Morifolium Ramat Chrysanthemum were determined by orthogonal test.The results showed that the ratio of material and solvent is 1∶10,enzyme treatment temperature was 45 ℃,pH was 6.2,cellulase dosage was 0.25 g/L,pectase dosage was 0.3 g/L,hydrolysis time was 150 min,extraction time was 60 min,volume of extracting solution was 40 mL.Under the conditions,the extraction rate of total flavone is up to 6.83%.

Key concepts: Chrysanthemum morifolium, Extraction (chemistry), Cellulase, Chromatography, Chemistry, Hydrolysis, Enzyme, Solvent

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