Establishment of rat fatty liver model induced by alcohol

Objective To establish a relatively simple and feasible rat model of alcoholic fatty liver.Methods Rat model of alcoholic fatty liver was induced by intragastric infusion of ethanol and high-fat diet for six weeks.Histopathological changes of live were assessed by hematoxylin and eosin staining.The activities of alanine transarninase(ALT) and aspartate aminotransferase(AST),the levels of total bilirubin(TBIL),total cholesterol(TC) and triglyceride(TG) in serum were detected with routine laboratory methods using an autoanalyzer.The activities of superoxide dismutase(SOD) and glutathione peroxidase(GPx) and the level of malondialdehyde(MDA) in liver homogenates were measured by spectrophotometry.The TG content in liver tissue was determined by spectrophotometry.Results At the end of the experiment,alcohol could induce liver injury as evidenced by aggravated steatosis,increased activity of ALT and levels of TBIL and TG in serum,reduced SOD and GPx activities and elevated MDA content in liver homogenates and TG content in liver tissue.Conclusion Alcoholic fatty liver model in rat was successfully established by alcohol gavage and high fat diet.