Breeding and field trial of a transgenic japonica rice line with resistance to brown planthopper and herbicide

Both GNA and Bar genes,located at the same binary vector pCUGNA-BAR,were introduced into a japonica rice variety Guanglingxiangjing by Agrobacterium-mediated transformation.Several transgenic rice plants were regenerated,and the GNA gene was integrated into the genome of transgenic plants confirmed by PCR and Southern blotting analyses.In the field trial,the selected homozygous transgenic rice line 3015-1-1 preserved advanced agronomic traits of its non-transformed wild type.The resistance to insect and herbicide were carefully analyzed,the results showed that the transgenic rice line 3015-1-1 showed significant inhibition to honeydew excretion and fecundity of brown planthopper,and high resistance to the herbicide Basta.