Application of Thin Prep cytology test and HPV testing in cervical lesions screening

Objective To assess the application value of Thin Prep cytology test( TCT) and high-risk HPV( HR-HPV) testing in cervical lesions screening. Methods A total of 1890 women who took routine cervical screening in The First People's Hospital of Hefei all underwent the TCT and HR-HPV testing by the Hibrid Capture 2( HC-2). Patients with positive results of TCT and / or HR-HPV were performed colposcopy and histological examination on the cervical tissue. Cross-check analysis was made of the results of TCT,HRHPV and pathology examination. Results 1The positive rate of HR-HPV increased along with the developing grade of abnormal TCT and cervical intraepithelial neoplasia( CIN) in patients. The HR-HPV infection rate of individuals with or = CINⅡ( including CINⅡ,CINⅢ,carcinoma in situ and invasive cancer) was significantly higher than CINⅠ or lower( P 0. 05). 2HR- HPV testing and TCT combined with HR- HPV testing were all substantially more sensitive in detecting or = CINⅡ than TCT( P 0. 05) but less specific( P 0. 05). The sensitivity of TCT combined with HR-HPV testing was slightly higher than HR-HPV testing in detecting or = CINⅡ( 92. 50% vs 82. 5%). 3Of the 105 cases with the cytological diagnosis of atypical squamous cell of undetermined significance( ASCUS),the pathologic diagnosis rate of or = CINⅡ in individuals with positive HR-HPV infection was significantly higher than in HRHPV negative group( P 0. 05). Conclusions 1HR-HPV testing as an adjunct to conventional cytology in cervical lesions screening seems to increase detection sensitivity with proven cost-effectiveness. It can improve detectable rate of cervical cancer and precancerous lesions. It has important significance for prevention and cure of cervical cancer. 2HR-HPV testing is favorable for shunting management in patients with TCT showing as ASCUS,but they should receive the TCT and HR-HPV testing regularly.