Expression of hCTR1,FHIT and PCNA in cervical intraepithelial neoplasia and cervical invasive squamous cell carcinoma and their clinical significance

Purpose To study the expression of human copper transporters 1(hCTR1),fragile histidine triad(FHIT) and proliferating cell nuclear antigen(PCNA) in cervical intraepithelial neoplasia(CIN) and cervical invasive squamous cell carcinoma(SCC) and investigate their correlativity in SCC.Methods hCTR1,FHIT and PCNA were examined by immunohistochemical staining(SP method) in 94 cases of CIN,40 cases of cervical invasive SCC,and 23 cases of normal cervical squamous epithelia.Results The positive rates of hCTR1 in SCC(87.50%) and in the level CIN 2~3(74.63%) were significantly higher than those of normal cervical squamous epitheliums(17.39%) and CIN 1(29.63%)(P0.001).In normal cervical squamous epithelium,FHIT positive rate was 100%.From normal cervical squamous epithelium to CIN(CIN 1,74.07%,CIN 2~3,44.78%) and SCC,FHIT expressed cells gradually decreased.The positive rates of FHIT were different between every group(P0.001).The expression of FHIT in the SCC and in CIN were significantly lower than those of normal cervical squamous epithelium(P0.001).The differences between CIN 2~3,SCC and CIN 1 were significant(P0.05).The expression of PCNA in SCC(75.00%) and in CIN 2~3(64.18%) were significantly higher than those of normal cervical squamous epithelium(26.09%)(P0.05,P0.001).The differences between CIN 2~3 and CIN 1 were significant(P0.05).There was a positive correlation between expression of FHIT and PCNA(P0.05,r=-0.377),but no correlation was found between hCTR1 and FHIT(P0.05,r=-0.296) or hCTR1 and PCNA(P0.05,r=0.026).Conclusions hCTR1 expression intensity and positive rate in cervical carcinoma in situ were higher than that in early invasive cancer in cervix,which may prove that there is a negative correlation between hCTR1 expression and malignant biological characteristics of invasiveness.There is a balance between FHIT and PCNA,which take part in the regulation of cell proliferation.Therefore,the detection of hCTR1 and FHIT expression plays an important role in screening and predicting high-grade CIN and SCC.