Expression and clinical significance of FHIT and PCNA protein in Cervical Squamous Cell Carcinoma and Cervical Intraepithelial Neoplasia

Objective:To study the expression of FHIT and PCNA in cervical squamous Cell carcinoma(SCC) and cervical intraepithelial neoplasia(CIN) and to investigate the role and significance of them.Methods:The expression of FHIT protein and PCNA protein was determined using immunohistochemistry(S-P) in 18 cases of normal cervical squamous epithelium,52 cases of CIN and 69 cases of cervical SCC.Results:The rates for loss of expression of FHIT genes in the squamous epithelium of normal cervices,CIN Ⅰ,CIN Ⅱ,CIN Ⅲand invasive cervical SCC were 0%,9.1%,29.4%,41.7%,66.7%.The comparison between normal cervical squamous epithelium(NEC) and cervical SCC,the comparison between CIN and cervical SCC,the comparison between CIN Ⅰ、Ⅱ and CIN Ⅲ have statistic significance(P0.05).Along with the decreasing of cell differentiation,the negative rate of FHIT raised.The positive expression of PCNA in cervical SCC group was 84.1%,higher than that in CIN and normal groups(P0.01).The positive rate of PCNA in the depleted expression of FHIT group was significantly higher than that in FHIT normal expression group(P0.01).There is a negative correlation between FHIT And PCNA expression.Conclusion:The FHIT-depletion and PCNA over-expression are related with cervical carcinogenesis.The FHIT-depletion may be a clinically useful marker of CIN more likely to progress to invasive disease;Depleted expression of FHIT is freguently associated with PCNA over-epression in CIN and cervical SCC subjects.There is a negative correlation between FHIT And PCNA expression.