Relationship Between Expression of FHIT Genes and HPV16/18 Infection in Cervical Squamous Cell Carcinoma and Cervical Intraepithelial Neoplasia

Objective To study the expression of human papilloma viruses 16 and 18(HPV16/18)DNA and FHIT genes in cervical intraepithelial neoplasia(CIN)and cervical carcinoma(CC)and investigate the role and significance of them.Methods Tumor samples were taken from 50 cases of CIN,and 50 cases of CC were processed by immunohistochemistry(SP)to determine the expression of FHIT genes,by in situ hybridization to detect HPV16/18 infection.Results The rates for loss of expression of FHIT genes in the squamous epithelium of normal cervices,CINⅠ,CINⅡ,CINⅢand invasive cervical squamous carcinoma were 0%(0/ 15),13.33%(2/15),33.33%(5/15),50%(10/20),and 78%(39/50)respectively.The comparison between Normal Cervi- cal Epithelium(NEC)and CC,the comparison between CIN and CC,the comparison between CINⅠand CINⅡ,CINⅢhave statistic significance(P0.05).Among the well differentiated,intermediately differentiated and poorly differentiated invasive cervical carci- noma,the rates for loss of expression of FHIT were 61.53(8/13),73.68%(14/19),and 94.44%(17/18)respectively(P0. 05).There was no significant difference between the loss of expression of FHIT and clinical stage,lymph node metastasis(P0.05).There was a positive correlation between FHIT expression and HPV16/18 infection in cervical squamous carcinoma(P0.05).Conclusions HPV16/18 infection and the FHIT-depletion are closely related with cervical carcinogenesis.The FHIT- depletion may be a clinically useful marker of CINs,more likely to progress to invasive disease.HPV16/18 infection may probably be the factor leading to altered FHIT expression.