Optimization of transplanted tumor models of chick embryo chorioallantoic membrane and biological characteristics of HepG2-CAM

AIM To compare four kinds of tumor strains model of chick embryo chorioallantoic membrane( CAM) in order to select the best strain and gain its biological characteristics. METHODS Four tumor strains,including Sarcoma S180,mouse hepato carcinoma call line H22,human lung cancer cell line A549,and human hepatocellar carcinoma cell line HepG2,were inoculated with CAM,and CAM survival,tumor survival,tumor formation,tumor-inducing angiogenesis and other indicators were observed. RESULTS The chicken embryo survival and tumor-inducing angiogenesis of the four groups had no significant differences,but human liver cancer HepG2 had the highest rate of tumor growth( P 0. 05),its strain was considered as an optimal xenografted tumor strain.CONCLUSION The best inoculation cell count of HepG2-CAM is 8 × 106/ chick embryo,and the growth cycle for the implanted tumor is 10th day and the best transplanting period is 8th day.