Improvement of chick embryo chorioallantoic membrane model of human tumor xenograft and observation of their biological behavior

Objective To establish a human tumor xenograft model on the chick embryo chorioallantoic membrane(CAM) and observe its biological behavior,and improve its modeling technique.Methods Human glioma cell line SWO-38 cells were implanted on the chick embryo chorioallantoic membrane to observe the factors affecting the survival of the transplanted human glioma cells,growth characteristics of the transplanteded tumor and their morphological features.Result Human glioma model was successfully established on the chick embryo chorioallantoic membrane,showing angiogenetic effect and morphological characteristics of glioma.Conclusions This model can be easily reproduced and may be used to observe biological behavior of the transplanted tumor and to screen antineoplastic drugs.