Establishment of Sarcoma S180 Model on Chick Embryo Chorioallantoic Membrane and Its Biological Properties

Objective:To establish S180 sarcoma model on the chick embryo chorioallantoic membrane(CAM) and to study its morphological and biological properties.Method: S180 cell was cultivated and implanted on the CAMs under sterile conditions.The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied.Result: S180 sarcoma model on the CAM was established.The implanted tumor was easy to grow on the CAM,and had a strong tendency of angiogenesis.When the number of inoculated tumor cells reached 5×107 in each egg,the tumor rate could reach 83.33%.Compared with the first day,the number of induced vessels on the second day was significantly different(P0.05).On the fourth day after transplant it was greatly increased(P0.001).Conclusion: Our findings suggest that this model is of easy duplication and simple operation,and can be widely used in the research of the biological properties for tumor angiogenesis and it provides an intuitive platform for the drug screening of anti-angiogenic.