Establishment of an implanted model of human pancreatic carcinoma on chick embryo and study of its tumor biology

Objective To establish an implanted model of pancreatic carcinoma on chick embryo and provide a useful and convenient tool for the study of pancreatic carcinoma. Methods Human pancreatic carcinoma cell line SW1990 was transplanted on the chick embryo chorioallantoic membrane(CAM). Developmental and biological characteristics of the implanted tumor were observed. Factors influencing neoplasia of pancreatic carcinoma cell were analyzed, and histology of the implanted tumor was investigated. Results The chick embryo implant model of human pancreatic carcinoma was established successfully. The histology characteristics of the implanted tumor were similar to those of human pancreatic carcinoma. The quantity of implanted tumor cell influenced the tumor survival rate: the more the tumor cells were implanted, the higher the survival rate was. The implanted tumor was able to induce tumor angiogenesis and the neonatal vessels grew concentrated to the tumor and formatted a radiated vessel network. Conclusions Human pancreatic carcinoma cell line SW1990 can be transplanted successfully on chick embryo CAM. This animal model can be used to observe the growth of pancreatic carcinoma continuously. As the implanted tumor can stimulate its growth pattern in human body, it would prove to be a useful model for the study of human pancreatic carcinoma.