2012•Zhongguo yaofangRequires access

Content Determination of Eucommiol in Eucommia ulmoides by RP-HPLC

Nanyin Han

Abstract

OBJECTIVE: To develop the method for the content determination of eucommiol in Eucommia ulmoides. METHODS: RP-HPLC method was adopted. The sample was separated on Chromasail C18(250 mm×4.6 mm,5 μm) column with methanol-water (8 ∶ 92) as the mobile phase at the flow rate of 1 mL·min-1. The column temperature was 25 ℃and the detection wavelength was set at 210 nm. RESULTS: The linear range of eucommiol was 0.467 6～5.845 0 μg(r=0.999 9) with an average recovery of 99.04%(RSD=2.16%,n=9). CONCLUSION: The method is simple, stable, controllable and repeatable, and it could be used for the content determination of encommiol in Eucommia ulmoide.

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Available abstract

Key concepts: Eucommia ulmoides, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Linear range, Linear relationship, Analytical Chemistry (journal)