Expression of Protein SOD、MDA in Aβ_(1-40) Hippocampus Injection-induced AD Rats and the Effect of TongluoJiu'nao Oral Solution

Objective To observe the effect of TLJN Oral Solution on protein SOD,MDA expression in brain tissue of amyloid-β1-40 injection into Hippocampus-induced experimental AD rats in vivo.Methods 140 SD rats were enrolled in the study,and they were equally randomly divided into blank control group,sham control group,negative control group,positive control group and experiment group(12,24,48 mg·kg-1·d-1).A rat model of AD was induced by amyloid-β1-40 sterotaxis injection into the bilateral hippocaupa.All rats were sacrificed in the 5th week after treatment for 4 weeks.The total activity of SOD and level of MDA in brain tissue were detected by ultraviolet-spectrophotometry.Results The SOD activity was decreased in model groups(P0.05)compared with the sham group and MDA level was increased.In experiment groups the SOD activity was increased and MDA content was reduced compared with the model group(P0.05).Conclusion TLJN Oral Solution can up-regulate expression of SOD and down-regulate expression of MDA.TLJN Oral Solution can inhibit the incidents of Alzheimer's disease by antioxidative effect.