Content determination of kaempferol in Jianyutangkang tablet by HPLC

Objective: To develop the method for quality control of kaempferol in Jianyutangkang tablet.Methods: Diamonsil-C18 column(250 mm × 4.6 mm,5 μm)was used for separation.The mobile phase consisted of acetonitrile-0.1% formic acid(33:67) with the flow rate of 0.8 mL·min-1,and the detection wavelength was set at 258 nm.Results: The kaempferol peak was separated from other peaks without interference by negative sample.The linear range of kaempferol was 2.076-20.76 μg·mL-1(r = 0.999 8).The recovery was 100.39% with RSD of 0.52%.Conclusion:The method is simple,accurate and repeatable,and it is suitable for the content determination of kaempferol in Jianyutangkang tablet.