Indirect Fluorescent Antibody Method for Fast Diagnosis of Pathogenic Bacteria of Emulsification Disease in Portunus trituberculatus

Presented in this paper is a method of Indirect of Fluorescent Antibody Technique (IFAT) for rapid diagnosis of pathogenic bacteria of emulsification disease in portunus.The antiserum for Vibrio alginolyticus was obtained by immunizing rabbit against Vibrio alginolyticus,the anti-rabbit IgG goat was marked fluresceinisothiocyanate and used in an IFAT detection.The result show that the lowest Vibrio alginolyticus suspension detection limit in laboratory was 10~5cfi/mL.In artificial infection,Vibrio alginolyticus was found after 24h.On worksite,Vibrio alginolyticus were noted by IFAT to be 90% and 20% for the clinically diseased and healthy Portunus trituberculatus,respectively.The results indicate that not only can the IFAT be applied to diagnose the infected Portunus;but also to detect those slightly infected.