STUDIES ON THE RAPID DETECTION OF Vibrio alginolyticus BY INDIRECT FLUORESCENT ANTIBODY STAINING

A rapid detection of Vibrio alginolyticus infecting the Large yellow croaker Pseudosciaena crocea by indirect fluorescent antibody staining has been developed. The specific antisera for V. alginolyticus was prepared with New Zealand rabbit,and cross reactive efficacy was tested by coagulation in tube. It seemed that the goat anti rabbit IgG had been marked by fluorescent isothiocyanate (FITC). The results showed that the positive reaction detected in situ from the typical symptom P. crocea infected by Vibrio was 100%,30% from the health ones also suffered by this pathogen , but negative detection from its aquaculture sea water. It demonstrated that the rapid detection by indirect fluorescent antibody could be not only used for testing the ill yellow croaker infected by pathogen Vibrio, but also for those healthy ones with little pathogen.