Establishment of a Vibrio alginolyticus Disease Model for the Crab, Portunus trituberculatus

Crabs,Portunus trituberculatus,were infected artificially with Vibrio alginolyticus with a concentration of 107 CFU·mL-1.The external symptoms and histopathological changes of infected crab were observed and the physio-biochemical indexes were determined at 0,24,48 and 72 h post-injection.The results indicated that the pathological changes of the infected crab were obvious gradually at 24 h post-injection and THCs、T-AOC、AKP、ACP and POD had significant changes,so the indexes can be used to determine whether the establishment of disease model was successful in this paper.The main objection of drug prevention was early diseased population,the time of establishment of disease model was at 24 h post-injection.The Vibrio alginolyticus disease model for Portunus trituberculatus which was established by this method was reproducible highly and convenient for observing changes of crabs.